CC-146 nr1-1 spr-u-1-6-2 mt-
$30.00
Boynton-Gillham laboratory, Duke University
Phenotype: antibiotic resistant (neamine, spectinomycin)
From a cross by Gillham of spr-u-1-6-2 mt+ x nr-u-2-1 sr-u-2-60 mt-, selected as a potential recombinant resistant to both spectinomycin and nearmine, but sensitive to streptomycin.
When outcrossed to wild type, the neamine resistance in this strain showed Mendelian segregation, and the strain was not cross-resistant to kanamycin (although the original nr-u-2-1 mutant is resistant) nor were chloroplast ribosomes resistant to neamine in poly-U directed amino acid incorporation. Therefore this strain contains a new spontaneous neamine-resistance mutation. Subsequently this was mapped to linkage group VIII and shown to be allelic with other nuclear nr1 mutants. This particular allele was designated nr1-1, and is the allele present in a number of derivative strains, including CC-975, CC-1088, CC-1144, CC-1714, CC-1720, CC-1730, CC-1735, and probably CC-2645.
This is a good strain to use either for spr-u-1-6-2 or nr1 in mt-.