CC-155 ac20 cr1 mt+
$30.00
Boynton-Gillham laboratory, Duke University
Phenotype: requires acetate; cold sensitive on minimal medium
Product 5.6.2 from a cross of wild type to the original ac20 cr1 double mutant (CC-154 mt-, a strain now discontinued.)
This strain was used by Boynton et al. (1972) in their study of mutants deficient in chloroplast ribosomes. This strain is a good choice when maximal deficiency in chloroplast protein synthesis is desired. Even so, this strain does not have a stringent acetate requirement, and does grow slightly on minimal medium. Scoring is easier at cool temperatures, e.g. 15 degrees.
Boynton JE, Gillham NW, Burkholder B (1970) Mutations Altering Chloroplast Ribosome Phenotype in Chlamydomonas, II. A New Mendelian Mutation. Proc Natl Acad Sci USA 67:1505-1512
Boynton JE, Gillham NW, Chabot JF (1972) Chloroplast ribosome deficient mutants in the green alga Chlamydomonas reinhardi and the question of chloroplast ribosome function. J Cell Sci 10:267-305