CC-1711 ery2a mt+
$30.00
Chlamydomonas Genetics Center, Duke University, 1983
Phenotype: antibiotic resistant (erythromycin)
The ery2a allele was obtained in Bogorad’s laboratory after mutagenesis of a 137c wild-type strain with ethyl methanesulfonate. CC-895 ery2a mt+ (which is no longer in the collection) was crossed to CC-124 wild type mt-, and a ery2a mt- product from this cross was crossed to CC-125 wild type mt+ to give CC-1711.
This strain provides better viability in crosses than the original CC-895. Since ery2 is easily scored and is a good marker for the sparsely populated linkage group XIV, having this in a strain that crosses well seemed desirable.
The ery2 mutants as a group are resistant to 100 micrograms/ml erythromycin on minimal medium, and are cold-sensitive.
Bowers et al. showed that the ERY2 locus corresponds to the gene encoding chloroplast ribosomal protein L22.
Mets LJ, Bogorad L (1971) Mendelian and uniparental alterations in erythromycin binding by plastid ribosomes. Science 174:707-709
Mets LJ, Bogorad L (1972) Altered chlorplast ribosomal proteins associated with erythromycin-resistant mutants in two genetic systems of Chlamydomonas reinhardi. Proc Natl Acad Sci U S A 69:3779-3783
Davidson JN, Hanson MR, Bogorad L (1978) Erythromycin resistance and the chloroplast ribosome in Chlamydomonas reinhardi. Genetics 89:281-297
Bowers AK, Keller JA, Dutcher SK (2003) Molecular markers for rapidly identifying candidate genes in Chlamydomonas reinhardtii. Ery1 and ery2 encode chloroplast ribosomal proteins. Genetics 164:1345-1353