CC-1722 ac209 mt-

$30.00

Chlamydomonas Genetics Center, Duke University, 1983

Phenotype: requires acetate (somewhat suppressed)

The ac209 mutation was UV-induced in Levine’s laboratory. This mutant was listed by Levine and Goodenough (1970) as nonphotosynthetic. The specific defect has not been determined.

From a cross at Duke in 1982. The ac209 marker in this strain ultimately traces back to CC-557 ac209 mt-, received from Ursula Goodenough in 1978 and no longer in the collection.

In a test cross in 1983, the ac209 marker was scorable only as a color difference rather than absolute acetate requirement, but was clearly segregating 2:2. This is the only ac209 isolate now in the collection.

The AC209 locus was originally placed on the left arm of linkage group I based on a cross in Levine’s laboratory of ac206 x ac14 (39:0:130). However, other crosses by Levine and colleagues, as well as crosses of ac209 isolates at the Chlamydomonas Genetics Center did not show the expected linkage to group I markers.

Levine RP, Goodenough UW (1970) The genetics of photosynthesis and of the chloroplast in Chlamydomonas reinhardi. Annu Rev Genet 4:397-408