CC-1815 rbcL (G171D) mt+ [Mets 10-6C]
$30.00
From Laurie Mets, April 1984, as III 130 10-6c; this is the original chloroplast Rubisco mutant 10-6C of Spreitzer and Mets.
Phenotype: requires acetate
This is a point mutation in the chloroplast rbcL gene (Gly to Asp at 171) that results in a Rubisco large subunit with altered isoelectric point.
This strain is light-sensitive and should be maintained in very dim light or in total darkness.
Spreitzer, Mets (1980) Non-mendelian mutation affecting ribulose-1,5-biphosphate carboxylase structure and activity. Nature 285:114-115
Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 67:565-569
Dron M, Rahire M, Rochaix JD, Mets L (1983) First DNA sequence of a chloroplast mutation: a missense alteration in the ribulosebisphosphate carboxylase large subunit gene. Plasmid 9:321-324
Spreitzer RJ, Ogren WL (1983) Rapid recovery of chloroplast mutations affecting ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 80:6293-6297
Gans P, Rebeille F (1988) Light inhibition of mitochondrial respiration in a mutant of Chlamydomonas reinhardtii devoid of ribulose-1,5-bisphosphate carboxylase/oxygenase activity. Arch Biochem Biophys 260:109-117
Rawat M, Henk MC, Lavigne LL, Moroney JV (1996) Chlamydomonas reinhardtii mutants without ribulose-1,5-bisphosphate carboxylase-oxygenase lack a detectable pyrenoid. Planta 198:263-270