* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-2420 lor1-101

Name: CC-2420 lor1-101
SKU: CC-2420
Availability: InStock

$30.00

From Alexander Chunayev, Leningrad State University, September 1989

Phenotype: carotenoid deficient; requires acetate

This strain should be kept in dim light.

The following notes on lor1-101 were sent by Armino Boschetti in 2001:
“The pg-101 [lor1] mutant was obtained by M. Tellenbach by UV mutation of C. reinhardtii strain 11/32f Levine, mutant Arg2 mt+ (from Culture Collection of Algae and Protozoa, Cambridge UK) and selected for altered color [M. Tellenbach (1984), Herbizidresistenz und Pigmentdefizienz, Ph.D. thesis, University of Bern, Switzerland]. The phenotypical difference detected at first was the lack in loroxanthin on thinlayer chromatograms of pigment extracts. As separation of lutein and zeaxanthin by HPLC as well as confirmation of the absence of low amounts of alpha-carotene is rather difficult, it took us some years to demonstrate that this mutant is not able to produce alpha-ionone rigns in carotenoids (episilon-rings), which means that the alpha cyclase is inactive or missing [A.S. Chunayev et al. (1991). Chlorophyll b- and loroxanthin-deficient mutants of Chlamydomonas reinhardtii, Photosynthetica 25, 291-301]. It was renamed to lor-1 by Chunayev who studied the genetics of this mutant. ”

Eichenberger W, Boschetti A, Michel HP (1986) Lipid and pigment composition of a chlorophyll b-deficient mutant of Chlamydomonas reinhardii. Physiologia Plantarum 66:589-594

Chunaev AS, Mirnaya ON, Maslov VG, Boschetti A (1991) Chlorophyll b- and loroxanthin-deficient mutants of Chlamydomonas reinhardtii. Photosynthetica 25:291-301

Niyogi KK, Björkman O, Grossman AR (1997) The roles of specific xanthophylls in photoprotection. Proc Natl Acad Sci U S A 94:14162-14167

Baroli I, Do AD, Yamane T, Niyogi KK (2003) Zeaxanthin accumulation in the absence of a functional xanthophyll cycle protects Chlamydomonas reinhardtii from photooxidative stress. Plant Cell 15:992-1008

Anwaruzzaman M, Chin BL, Li XP, Lohr M, Martinez DA, Niyogi KK (2004) Genomic analysis of mutants affecting xanthophyll biosynthesis and regulation of photosynthetic light harvesting in Chlamydomonas reinhardtii. Photosynth Res 82:265-276

CC-2420 lor1-101

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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