CC-2453 nit1-305 mt-
$30.00
From P.A. Lefebvre, University of Minnesota, January 1990
The nit1-305 mutation was isolated by Sosa et al. in strain 21 gr (see CC-1690), using nitrosoguanidine mutagenesis and selection for chlorate resistance. This nit1 allele is specifically deficient in the diaphorase activity of the nitrate reductase complex. Fernandez and Cardenas reported that the diaphorase subunit had significantly lower molecular weight than its wild type equivalent. This mutation can be complemented by transformation with the cloned NIT1 gene. This strain has the wild type allele at the NIT2 locus.
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