* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-2485 dd-m-112 mt-
$30.00
From Gianni Piperno, Rockefeller University, June 1990
Phenotype: impaired motility
This mutant was isolated by nitrosoguanidine mutagenesis of cells grown in synchronous cultures (12:12 light-dark), at a time approximately 2 hours into the dark cycle. It has a temperature-conditional defect in flagellar function. Cells resemble type at permissive temperature (20 degrees) but when shifted to 32 degrees, the flagella beat in an rapid, uncoordinated manner. Wild-type function is recovered with a shift to permissive temperature, and does not require reassembly.
Huang B, Rifkin MR, Luck DJ (1977) Temperature-sensitive mutations affecting flagellar assembly and function in Chlamydomonas reinhardtii. J Cell Biol 72:67-85