* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-2488 dd-l-108 mt+

Name: CC-2488 dd-l-108 mt+
SKU: CC-2488
Availability: InStock

$30.00

From Gianni Piperno, Rockefeller University, June 1990

Phenotype: impaired motility

This mutant was isolated by nitrosoguanidine mutagenesis of cells grown in synchronous cultures (12:12 light-dark), at a time approximately 2 hours into the dark cycle. It has a temperature-conditional defect in flagellar function. Cells resemble wild type at permissive temperature (20 degrees) but when shifted to 32 degrees, the flagella detach rapidly. Cells can initiate flagellar reassembly at 32 degrees, but as the flagella elongate they are lost by detachment.

Cells of this mutant cannot form colonies at 32 degrees under constant light, where rapid growth and cell division are required. Cells grown on an alternating 12 h light-dark cycle divide normally.

Huang B, Rifkin MR, Luck DJ (1977) Temperature-sensitive mutations affecting flagellar assembly and function in Chlamydomonas reinhardtii. J Cell Biol 72:67-85

CC-2488 dd-l-108 mt+

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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