CC-2495 pf22 mt+
$30.00
From Gianni Piperno, Rockefeller University, June 1990
Phenotype: impaired motility
This mutation was induced with nitrosoguanidine in Luck’s laboratory, and is allelic with pf22A, isolated at the same time. The pf22 mutants partially lack outer dynein arms of axonemal doublet microtubules; flagella are short in exponentially growing cells, about two-thirds of wild type length at stationary phase, and are deficient in 14 axonemal polypeptides.
Huang B, Piperno G, Luck DJ (1979) Paralyzed flagella mutants of Chlamydomonas reinhardtii. Defective for axonemal doublet microtubule arms. J Biol Chem 254:3091-3099
Okagaki T, Kamiya R (1986) Microtubule sliding in mutant Chlamydomonas axonemes devoid of outer or inner dynein arms. J Cell Biol 103:1895-1902
Kamiya R (1995a) Exploring the function of inner and outer dynein arms with Chlamydomonas mutants. Cell Motil Cytoskeleton 32:98-102
Frey E, Brokaw CJ, Omoto CK (1997) Reactivation at low ATP distinguishes among classes of paralyzed flagella mutants. Cell Motil Cytoskeleton 38:91-99