* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-253 cr7 mt+
$30.00
Elizabeth Harris, Boynton-Gillham laboratory, Duke University
Phenotype: cold sensitive on minimal medium
This mutant accumulates some 41S and some 54S subunits of chloroplast ribosomes, or poorly defined mixed subunit material, and is very deficient in 70S ribosomes.
Myers et al. showed that cr7 and its allele cr6 fail to assemble to proteins of the large subunit of the chloroplast ribosome, one normally synthesized in the chloroplast and one in the cytoplasm.
Myers AM, Harris EH, Gillham NW, Boynton JE (1984) Mutations in a nuclear gene of Chlamydomonas cause the loss of two chloroplast ribosomal proteins, one synthesized in the chloroplast and the other in the cytoplasm. Curr Genet 8:369-378