* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-2653 rbcL (nonsense mutation) [Spreitzer 18-7G]
$30.00
From Robert Spreitzer, University of Nebraska, to N.W. Gillham and J.E. Boynton, added to public colllection 1991
Phenotype: requires acetate
This is Spreitzer’s strain 18-7G-12. The mutation is a UGG > UAG change near the 5′ end of the gene, resulting in a 65-aa polypeptide.
Spreitzer RJ, Goldschmidt-Clermont M, Rahire M, Rochaix JD (1985) Nonsense mutations in the Chlamydomonas chloroplast gene that codes for the large subunit of ribulosebisphosphate carboxylase/oxygenase. Proc Natl Acad Sci U S A 82:5460-5464
Yu W, Spreitzer RJ (1992) Chloroplast heteroplasmicity is stabilized by an amber-suppressor tryptophan tRNA(CUA). Proc Natl Acad Sci U S A 89:3904-3907