CC-267 sr-u-sm5 mt+
$30.00
From Ruth Sager, Hunter College, March 1975
Phenotype: antibiotic resistant (streptomycin)
This is the primary stock of sr-u-sm5 in the Chlamydomonas Resource Center collection. Other streptomycin resistance mutations, notably sr-u-2-60, have been more frequently used in crosses for chloroplast genetics, however. This mutant has an alteration in the ’912′ region of the 16S rRNA molecule (A -> C at nt 858, corresponding to E. coli 914).
Like most of Sager’s strains, CC-267 grows on nitrate as sole N source (indicated by the nit+ or NIT allele designation). This property is in contrast to most strains from the Ebersold/Levine background, which carry the nit1 and nit2 mutations.
Sager R and Ramanis Z (1976) Chloroplast genetics of chlamydomonas. I. Allelic segregation ratios. Genetics 83:303-321
Harris EH, Burkhart BD, Gillham NW, Boynton JE (1989) Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: correlation of genetic and physical maps of the chloroplast genome. Genetics 123:281-292