CC-2681 colR4 22a mt-
$30.00
From Bessie Huang, Research Institute of Scripps Clinic, October 1991
Phenotype: herbicide / colchicine resistant (amiprophos methyl, oryzalin, colchicine)
This mutant is resistant to colchicine, vinblastine, dinitroanilines (e.g. oryzalin, trifluralin), and amiprophos methyl, and shows increased sensitivity to taxol. The mutation changes Lys350 of the beta-2 tubulin gene to Glu.
Bolduc C, Lee VD, Huang B (1988) Beta-tubulin mutants of the unicellular green alga Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A 85:131-135
Lee VD, Huang B (1990) Missense mutations at lysine 350 in beta 2-tubulin confer altered sensitivity to microtubule inhibitors in Chlamydomonas. Plant Cell 2:1051-1058
Schibler MJ, Huang B (1991) The colR4 and colR15 beta-tubulin mutations in Chlamydomonas reinhardtii confer altered sensitivities to microtubule inhibitors and herbicides by enhancing microtubule stability. J Cell Biol 113:605-614