CC-2702 cia5 mt+
$30.00
From James Moroney, Louisiana State University, April 1992
Phenotype: altered CO2 assimilation
This strain requires 5% CO2 for growth on minimal medium. In contrast to other cia mutants, cia5 fails to synthesize any of the components of the CO2 concentrating system, including the periplasmic carbonic anhydrase, when switched to an environment low in CO2. This is a mutation in the CCM1 gene, which has been identified as the “master regulator” of the carbon concentrating mechanism in Chlamydomonas.
Moroney JV, Husic HD, Tolbert NE, Kitayama M, Manuel LJ, Togasaki RK (1989) Isolation and Characterization of a Mutant of Chlamydomonas reinhardtii Deficient in the CO(2) Concentrating Mechanism. Plant Physiol 89:897-903
Marek LF, Spalding MH (1991) Changes in Photorespiratory Enzyme Activity in Response to Limiting CO(2) in Chlamydomonas reinhardtii. Plant Physiol 97:420-425
Spalding MH, Winder TL, Anderson JC, Geraghty AM, Marek LF (1991) Changes in protein and gene expression during induction of the CO2-concentrating mechanism in wild-type and mutant Chlamydomonas. Can J Bot 69:1008-1016
Xiang Y, Zhang J, Weeks DP (2001) The Cia5 gene controls formation of the carbon concentrating mechanism in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A 98:5341-5346
Fukuzawa H, Miura K, Ishizaki K, Kucho KI, Saito T, Kohinata T, Ohyama K (2001) Ccm1, a regulatory gene controlling the induction of a carbon-concentrating mechanism in Chlamydomonas reinhardtii by sensing CO2 availability. Proc Natl Acad Sci U S A 98:5347-5352