CC-28 ac17 can1 nic13 pf2 y1 pyr1 msr1 act2 sr1 mt+
$30.00
From W.T. Ebersold, UCLA, to Boynton-Gillham laboratory, Duke University, 1973
Phenotype: requires acetate and nicotinamide; resistant to antibiotics and metabolic inhibitors (canavanine, cycloheximide, methionine sulfoximine, pyrithiamine, and streptomycin)
CC-28 and CC-29 are a very useful pair of mapping strains, prepared by Smyth, Ebersold and colleagues. Both strains usually give complete tetrads in crosses, and the markers are generally easy to score.
The ac17 mutation (see CC-530) confers a very clean acetate requirement, easily scored by growth on minimal medium (plus nicotinamide in this strain because of the nic13 mutation).
The pf2 mutation (see CC-1025) should be scored as slow-swimming compared to wild type, and nic13 (see CC-864) should be scored by sensitivity to 3-acetyl pyridine rather than by requirement for nicotinamide.
The resistance mutations in this strain are scored as follows:
act2 (see CC-1590), resistant to about 10 micrograms/ml cycloheximide
can1 (see CC-863), resistant to 0.5 mg/ml canavanine sulfate
msr1 (first described by Sager, 1955), resistant to 500 micrograms/ml methionine sulfoximine
pyr1 (first described in by Smyth et al., cited here), resistant to 1 microgram/ml pyrithiamine in the absence of thiamine.
sr1 (see CC-112), resistant to 50 micrograms/ml streptomycin
The original strain also carried y1, but this needs to be confirmed before use due to its high interconversion rate with its wild type allele.
Sager R (1955) Inheritance in the Green Alga Chlamydomonas Reinhardi. Genetics 40:476-489
Smyth RD, Martinek GW, Ebersold WT (1975) Linkage of six genes in Chlamydomonas reinhardtii and the construction of linkage test strains. J. Bacteriol. 124:1615-1617