CC-2843 w-7
$30.00
From Marilyn Kobayashi, University of California Berkeley, Niyogi lab, November 2008
The w-7 mutant was obtained by Spreitzer and Mets after 5-fluorodeoxyuridine/ ethyl methanesulfontate mutagenesis of their strain 2137 (see CC-3269). It is very deficient in chloroplast pigments, white to pale green in color, and extremely light-sensitive. Our original stock was obtained from John Sullivan at the University of Michigan Kellogg Eye Center in 1992. He had received it from Wei-Yeh Wang at Iowa State University. That stock was eventually lost, and was replaced by an equivalent strain by Marilyn Kobayashi in the Niyogi laboratory, UC Berkeley, in 2008.
This mutant was mapped by Spreitzer and Mets to linkage group XI, and subsequently shown to be ian allele at the LTS1 locus, which in turn was identified as the PSY1 gene encoding phytoene synthase.
Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol. 67:565-569
Iroshnikova GA, Rakhimberdieva MG, Karapetyan NV (1982) Study of pigmentation-modifying mutations in strains of Chlamydomonas reinhardii of different ploidy. III. Characteristics of disturbances of the photosynthetic apparatus in the presence of mutations in the lts1 locus. Soviet Genetics 18:1350-1356
McCarthy SS, Kobayashi MC, Niyogi KK (2004) White mutants of Chlamydomonas reinhardtii are defective in phytoene synthase. Genetics 168:1249-1257