CC-2857 DCMU4 er-u-1a C. smithii / C. reinhardtii hybrid mt-

$30.00

Boynton-Gillham laboratory, Duke University

Phenotype: antibiotic and herbicide resistant (DCMU, erythromycin)

This is Scott Newman’s strain T64 P344/1852 dr er #10. It was derived from transformation of strain CC-1852, a hybrid strain containing the C. smithii chloroplast genome, with plasmid P-344, which is no longer in the Chlamydomonas Resource Center collection. This plasmid contained a 6.9 kb Eco-Sal C. reinhardtii insert running from psbA intron 4 to the 3′ portion of the 23S rRNA gene). Exon 5 of psbA contains the DCMU4 mutation and the rRNA gene contains the er-u-1a mutation. Two diagnostic RFLP fragments in the interval between psbA 5′ and rRNA 3′ were confirmed to have been derived from C. reinhardtii, rather than the C. smithii chloroplast genome of CC-1852 (see Newman et al. 1990, Figure 1).

Newman SM, Boynton JE, Gillham NW, Randolph-Anderson BL, Johnson AM, Harris EH (1990) Transformation of chloroplast ribosomal RNA genes in Chlamydomonas: molecular and genetic characterization of integration events. Genetics 126:875-888