CC-2887 psbA-RK (R238K)
$30.00
Amnon Lers, Boynton-Gillham laboratory, Duke University
This is Lers strain RK2. It contains a site-directed mutation that changes Arg238 of the psbA gene to Lys. This mutation occurs naturally in the D1 protein of the plant Phalaris paradoxa (Schoenfeld et al. 1987, Plant Physiol. 83, 329-333), where it appears to suppress negative effects on photosynthesis and growth produced by the triazine-resistant Ser264-> Gly mutation. Heifetz et al. reported that otherwise isogenic Chlamydomonas strains containing the DCMU4 (Ser264-> Ala) mutation alone and DCMU4 in combination with psbA-RK were indistinguishable with respect to various parameters of photosynthesis and growth rate.
Heifetz PB, Lers A, Turpin DH, Gillham NW, Boynton JE, Osmond CB (1997) Dr and spr/sr mutations of Chlamydomonas reinhardtii affecting D1 protein function and synthesis define two independent steps leading to chronic photoinhibition and confer differential fitness. Plant Cell Environ 20:1145-1157