CC-2958 arg9-2 shf1 mt-
$30.00
From Patrick Ferris, Goodenough lab, Washington University, December 1993
Phenotype: requires arginine; short flagella
The arg9 mutants are deficient in acetylornithine aminotransferase and require arginine, citrulline or ornithine for growth. The ARG9 locus was mapped to a position about 20 cM centromere-proximal to the mating type locus by Ferris and Goodenough.
Note from Ferris sent with stock: “This died on plain TAP, confirming arg. It appears to have shorter flagella than wild type, but I’m never sure I can accurately judge this, so I checked the ‘bald in acetate-containing media’ phenotype of shf1 (which it has). The mt checks out also. This was constructed as follows. We got arg9-2 mt+ from Loppes, shf1-253 mt- from Jarvik [see CC-2348]. These were crossed to yield an arg9-2 shf-1 mt+ (since lost). This strain was crossed to CC-124 to create the arg9-2 shf-1 mt-.”
Loppes R, Heindricks R (1986) New arginine-requiring mutants in Chlamydomonas reinhardtii.
Arch Microbiol 143:348-352
Ferris PJ, Goodenough UW (1994) The mating-type locus of Chlamydomonas reinhardtii contains highly rearranged DNA sequences. Cell 76:1135-1145