* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-2959 agl1 arg2 msr1 spr-u-1-27-3 mt-
$30.00
From Patrick Ferris, Goodenough lab, Washington University, December 1993; originally from Matsuda
Phenotype: does not mate; requires arginine; antibiotic and inhitibor resistant (spectinomycin, methionine sulfoximine)
This is the original agl1 mutation induced in Matusda’s strain 7457B (arg2 spr-u-1-27-3 msr1). This mutation is an allele at the sex-linked SAD1 locus on linkage group VI. Mutants at this locus do not agglutinate with mt+ cells.
Matsuda Y, Saito T, Umemoto T, Tsubo Y (1988) Transmission patterns of chloroplast genes after polyethylene glycol-induced fusion of gametes in non-mating mutants of Chlamydomonas reinhardtii
Curr Genet 14:53-58