CC-3022 atpC1 nit1-305 cw15 mt-
$30.00
From Stuart Ross in Bruce Selman’s lab, University of Wisconsin, December 1994
Phenotype: requires acetate; wall deficient
This is a null mutation in the ATPC gene encoding the gamma subunit of CF1 ATP synthase. The mutant was obtained by Eric Smart by transformation of a nit1-305 cw15 strain with a construct in which a 168 bp Bgl II fragment from the ATPC cDNA was replaced with a plasmid sequence. Transformants were selected for arsenate tolerance and screened for an acetate-requiring phenotype. The mutant was shown to have a defect in the 5′ portion of the AtpC gene. It does not accumulate either mRNA or a polypeptide.
For site-directed mutations that alter specific amino acids in ATPC, please see CC-3023 through CC-3027.
Smart EJ, Selman BR (1991) Isolation and characterization of a Chlamydomonas reinhardtii mutant lacking the gamma-subunit of chloroplast coupling factor 1 (CF1). Mol Cell Biol 11:5053-5058
Smart EJ, Selman BR (1993) Complementation of a Chlamydomonas reinhardtii mutant defective in the nuclear gene encoding the chloroplast coupling factor 1 (CF1) gamma-subunit (atpC). J Bioenerg Biomembr 25:275-284
Ross SA, Zhang MX, Selman BR (1996) A role for the disulfide bond spacer region of the Chlamydomonas reinhardtii coupling factor 1 gamma-subunit in redox regulation of ATP synthase. J Bioenerg Biomembr 28:49-57
Ponomarenko S (2006) Algae cells with deletion of the segment D210-R226 in γ subunit from chloroplast ATP synthase have lower transmembrane proton gradient and grow slowly. Photosynthetica 44:338-344