* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-3026 atpC (D199A) nit1-305* cw15 mt-
$30.00
From Stuart Ross in Bruce Selman’s lab, University of Wisconsin, December 1994
Phenotype: requires acetate
This mutation was obtained by co-transformation of atpC1 nit1-305 cw15 (CC-3022) with pMN24 (containing the wild type gene for NIT1) and a construct containing a mutant AtpC gene. This mutation substitutes Ala for Asp at position 199 of the CF1 gamma subunit polypeptide.
Ross SA, Zhang MX, Selman BR (1996) A role for the disulfide bond spacer region of the Chlamydomonas reinhardtii coupling factor 1 gamma-subunit in redox regulation of ATP synthase. J Bioenerg Biomembr 28:49-57