* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-3027 atpC (D199K/K203D) nit1-305* cw15 mt-
$30.00
From Stuart Ross in Bruce Selman’s lab, University of Wisconsin, December 1994
Phenotype: requires acetate
This ATPC mutation was obtained by co-transformation of atpC1 nit1-305 cw15 (CC-3022) with pMN24 (containing the wild type gene for NIT1) and a construct containing a mutant ATPC gene. This mutation substitutes Ala for Asp at position 199 and Lys for Asp at position 203 of the CF1 gamma subunit polypeptide.
Ross SA, Zhang MX, Selman BR (1996) A role for the disulfide bond spacer region of the Chlamydomonas reinhardtii coupling factor 1 gamma-subunit in redox regulation of ATP synthase. J Bioenerg Biomembr 28:49-57