* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-3055 KK5C12 mt+ [high tubulin expression]
$30.00
From Keith Kozminski in Joel Rosenbaum’s laboratory, Yale University, April 1995
This strain expresses epitope-tagged alpha1-tubulin to a high and constant level. It was created by transformation in March 1991. nit1-305 cells were cotransformed with pMN24 (nitrate reductase) and a construct containing the Chlamydomonas alpha1-tubulin gene tagged with a nine amino acid epitope from influenza virus hemagglutinin (HA). To obtain a high level of expression, the alpha1-tubulin promoter was replaced with the Rubisco S2 promoter.
Kozminski KG, Diener DR, Rosenbaum JL (1993) High level expression of nonacetylatable alpha-tubulin in Chlamydomonas reinhardtii. Cell Motil Cytoskeleton 25:158-170