CC-315 dk-104 mt-

$30.00

Andrew Wiseman, Boynton-Gillham laboratory, Duke University, 1977

Phenotype: dies in the dark

Isolated by Andrew Wiseman in the Boynton-Gillham laboratory, nitrosoguanidine-induced in wild type mt-. This is the original mutant.

This strain was originally described as respiratory deficient, unable to grow on acetate in the dark. It showed non-Mendelian segregation, suggestive of a possible mitochondrial mutation, but was not analyzed in detail because Wiseman was unable to obtain a homoplasmic stock with a consistent phenotype. Subsequently the original strain acquired what appears to be a secondary nuclear mutation. At last check, the strain still showed a dark-dier phenotype.


Wiseman A, Gillham NW, Boynton JE (1977) The mitochondrial genome of Chlamydomonas. II. Genetic analysis of non-mendelian obligate photautotrophic mutants. Mol Gen Genet 150:109-118