CC-3318 oda9-V27 mt+
$30.00
From George Witman, Worcester Foundation for Experimental Biology, January 1996
Phenotype: impaired motility
This mutant was generated by insertional mutagenesis of a nit1 agg1 mt+ parental strain, transformed with the plasmid pGP505 containing the wild-type NIT1 allele (see Pazour et al. 1995). NIT+ strains having a slow-swimming, jerky-swimming phenotype were selected. (This phenotype is typical of mutants lacking the outer dynein arms).
In this strain, outer dynein arms are absent, and the ODA9 gene is deleted (see Wilkerson et al., Figure 8). The NIT+ phenotype can be segregated away from the mutation Thus, derivatives of this strain would be useful for further transformations using NIT1 as a selectable marker. The genome contains pUC119 but it is not known if the plasmid sequence segregates with the deletion. This mutation does not complement oda9 in diploids.
Pazour GJ, Sineshchekov OA, Witman GB (1995) Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii. J Cell Biol 131:427-440
Wilkerson CG, King SM, Koutoulis A, Pazour GJ, Witman GB (1995) The 78,000 M(r) intermediate chain of Chlamydomonas outer arm dynein isa WD-repeat protein required for arm assembly. J Cell Biol 129:169-178