CC-3320 oda4-V50 mt+
$30.00
From George Witman, Worcester Foundation for Experimental Biology, January 1996
Phenotype: impaired motility
This mutant was generated by insertional mutagenesis of a nit1 agg1 mt+ parental strain, transformed with the plasmid pGP505 containing the wild-type NIT1 allele (see Pazour et al. 1995). NIT+ strains having a slow-swimming, jerky-swimming phenotype were selected. (This phenotype is typical of mutants lacking the outer dynein arms).
In this strain, the beta dynein heavy chain (ODA4) is deleted (see Figure 8 of Wilkerson et al. 1995). This deletion includes the 5′ end but not the 3′ end. The genome does not contain pUC119. It is not known if the NIT+ phenotype segregates with the deletion.
Pazour GJ, Sineshchekov OA, Witman GB (1995) Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii. J Cell Biol 131:427-440
Wilkerson CG, King SM, Koutoulis A, Pazour GJ, Witman GB (1995) The 78,000 M(r) intermediate chain of Chlamydomonas outer arm dynein isa WD-repeat protein required for arm assembly. J Cell Biol 12:169-178