* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-3353 far1-3 nit1-1 pf14 T(NIT1 NIT1/pf14) mt+ [Zhang SW7]
$30.00
From Donghong Zhang, Washington University, March 1997
The far1 (free from ammonia repression) mutants were isolated by insertional mutagenesis or by selection of spontaneous mutants with constitutive expression of the NIT1 (nitrate reductase) gene in the presence of ammonia. CC-3353 is the far1-3 mutant, which was isolated in a pf14 background after transformation with the wild-type RPS3 (PF14) gene as a reporter for expression from the NIT1 promoter).
Zhang D, Lefebvre PA (1997) FAR1, a negative regulatory locus required for the repression of the nitrate reductase gene in Chlamydomonas reinhardtii. Genetics 146:121-133