CC-339 brs1 r-1 mt+
$30.00
Jean Forster, Boynton-Gillham laboratory, Duke University, 1976; originally isolated by Wei-Yeh Wang. Original stock lost, replaced by Wang in December 1982 and again in February 1984
Phenotype: chlorophyll deficient, accumulates protoporphyrin
The brs1 mutant, which was UV-induced by Wei-Yeh Wang in strain CC-125, is chlorophyll-deficient and accumulates protoporphyrin IX. It is very light sensitive and must be grown in the dark. Chekounova et al. determined that the brs1 mutation is a frameshift in exon 10 of the CHLH gene.
Cultures of the original brs1 mutant were brown. The r1 mutation enhances the accumulation of protoporphyrin. This strain appears dark brown and is very light-sensitive.
Wang WY, Wang WL, Boynton JE, Gillham NW (1974) Genetic control of chlorophyll biosynthesis in Chlamydomonas. Analysis of mutants at two loci mediating the conversion of protoporphyrin-IX to magnesium protoporphyrin. J Cell Biol 63:806-823
Wang WY (1978) Genetic control of chlorophyll biosynthesis in Chlamydomonas reinhardtii. Int. Rev Cytol suppl 8:335-364
Johanningmeier U and Howell SH (1984) Regulation of light-harvesting chlorophyll-binding protein mRNA accumulation in Chlamydomonas reinhardi. Possible involvement of chlorophyll synthesis precursors. J Biol Chem 259:13541-13549
Chekounova E, Voronetskaya V, Papenbrock J, Grimm B, Beck CF (2001) Characterization of Chlamydomonas mutants defective in the H subunit of Mg-chelatase. Mol Gen Genet 266:363-373
Von Gromoff ED, Alawady A, Meinecke L, Grimm B, Beck CF (2008) Heme, a plastid-derived regulator of nuclear gene expression in Chlamydomonas. Plant Cell 20:552-567