CC-3460 chlL (aadA disruption) mt+
$30.00
Heriberto Cerutti in Boynton-Gillham laboratory, Duke University, August 1997
Phenotype: chlorophyll deficient
This strain was derived as follows:
CC-373, an atpB deletion mutant, was transformed with the wild-type Chlamydomonas atpB gene to produce CC-3452, which was used as a control for CC-3453, CC-3454, and CC-3455 containing the same atpB gene upstream of three versions of the E. coli recA gene. (These strains are no longer in the collection.)
CC-3452 was then crossed to CC-1930 arg2 mt-, and a wild-type progeny clone (i.e. not requiring arginine) was seleted, to yield CC-3456.
CC-3456 was then transformed with an aadA construct disrupting the chlL gene (Goldschmidt-Clermont), flanked by direct repeats to evaluate recombination frequencies in the chloroplast. CC-3460 is one of the transformants recovered.
Cerutti H, Johnson AM, Boynton JE, Gillham NW (1995) Inhibition of chloroplast DNA recombination and repair by dominant negative mutants of Escherichia coli RecA. Mol Cell Biol 15:3003-3011