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CC-3475 10-P (300) RbcS2/aadA/RbcS2 mt-

Name: CC-3475 10-P (300) RbcS2/aadA/RbcS2 mt-
SKU: CC-3475
Availability: InStock

$30.00

Heriberto Cerutti in Boynton-Gillham laboratory, Duke University, August 1997

This strain was derived by transformation of CC-124 wild type mt- with an RbcS2::aadA::RbcS2 construct (P-681) as described by Cerutti et al. (1997a). CC-3475 was characterized as having a stable spectinomycin resistance phenotype. It was fast growing, with an estimated number of 4 copies of the transforming aadA construct.

Cerutti H, Johnson AM, Gillham NW, Boynton JE (1997) A eubacterial gene conferring spectinomycin resistance on Chlamydomonas reinhardtii: integration into the nuclear genome and gene expression. Genetics 145:97-110

Cerutti H, Johnson AM, Gillham NW, Boynton JE (1997) Epigenetic silencing of a foreign gene in nuclear transformants of Chlamydomonas. Plant Cell 9:925-945

Locus:
RBCS2
Chromosome:

CC-3475 10-P (300) RbcS2/aadA/RbcS2 mt-

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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