CC-3672 B4(1) mt-?

$30.00

From Eli Greenbaum, Oak Ridge National Laboratory, July 1998

Phenotype: requires acetate

B4 is a mutant deficient in Photosystem I, isolated by treatment with 5-fluorodeoxyuridine followed by metronidazole selection as described by Roitgrund and Mets, 1990. It was characterized by Greenbaum et al. as a nuclear mutation that affects splicing of the chloroplast-encoded psaA gene.


Roitgrund C, Mets LJ (1990) Localization of two novel chloroplast genome functions: trans-splicing of RNA and protochlorophyllide reduction. Curr Genet 17:147-153

Greenbaum E, Lee JW, Tevault CV, Blankinship SL, Mets LJ (1995) CO2 fixation and photoevolution of H2 and O2 in a mutant of Chlamydomonas lacking Photosystem I. Nature 376:438-441

Lee JW, Tevault CV, Owens TG, Greenbaum E (1996) Oxygenic Photoautotrophic Growth Without Photosystem I. Science. 273:364-367

Greenbaum E, Lee JW, Tevault CV, Blankinship SL, Owens TG, Mets LJ (1997) Photosystem-I Measurements in Mutants B4 and F8 of Chlamydomonas. Science 277:166-167

Cournac L, Redding K, Bennoun P, Peltier G (1997) Limited photosynthetic electron flow but no CO2 fixation in Chlamydomonas mutants lacking photosystem I. FEBS Lett. 416:65-68