CC-3742 petA (K58E-K65E-K66E)
$30.00
From Michael Ponamarev and William Cramer, Purdue University, July 1999
Phenotype: requires acetate
The petA gene in this strain contains the indicated site-directed mutations.
Ponamarev MV, Cramer WA (1998) Perturbation of the internal water chain in cytochrome f of oxygenic photosynthesis: loss of the concerted reduction of cytochromes f and b6. Biochemistry 37, 17199-17208
Soriano GM, Ponamarev MV, Tae GS, Cramer WA (1996) Effect of the interdomain basic region of cytochrome f on its redox reactions in vivo. Biochemistry 35:14590-14598
Soriano GM, Ponamarev MV, Piskorowski RA, Cramer WA (1998) Identification of the basic residues of cytochrome f responsible for electrostatic docking interactions with plastocyanin in vitro: relevance to the electron transfer reaction in vivo. Biochemistry 37:15120-15128