CC-3766 psbA (A251L) mt+
$30.00
Anita Lardans, Boynton-Gillham laboratory, Duke University, 1999
Phenotype: requires acetate; herbicide resistant (atrazine, bromacil, metribuzin)
This strain was derived from a cross of CC-3382 “Leu suppressor mt+” (no longer in the collection) to CC-124 wild type mt-. The submission notes for CC-3766 describe it as psbA A251L. We believe that this is the same site-directed A251L mutation as is present in CC-3389, but that CC-3766 does not have the silent T247C mutation that is also in that strain. Please see CC-3389 for more information on this mutation.
Lardans A, Gillham NW, Boynton JE (1997) Site-directed mutations at residue 251 of the photosystem II D1 protein of Chlamydomonas that result in a nonphotosynthetic phenotype and impair D1 synthesis and accumulation. J Biol Chem 272:210-216