CC-377 clr-u-1 mt+
$30.00
From Ruth Sager, Sidney Farber Cancer Institute, December 1976
Phenotype: antibiotic resistant (clindamycin, erythromycin)
This mutant was isolated by Sager in her strain 21 gr background, as resistant to 50-100 micrograms/ml clindamycin (Cleocin), and designated by her as cle1 or cle. This mutant is cross-resistant to erythromycin, and was found by Bartlett et al. to be genetically allelic with the er-u-37 mutant. Harris et al. determined that this mutation is an A -> G change in the peptidyl transferase region of the 23S rRNA gene, at the base equivalent to E. coli 2058. The base pair change in this mutant is identical to that in the independently isolated erythromycin-resistant mutant er-u-AW-17.
Bartlett SG, Harris EH, Grabowy CT, Gillham NW, Boynton JE (1979) Ribosomal subunits affected by antibiotic resistance mutations at seven chloroplast loci in Chlamydomonas reinhardtii. Mol Gen Genet 176:199-208
Harris EH, Burkhart BD, Gillham NW, Boynton JE (1989) Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: correlation of genetic and physical maps of the chloroplast genome. Genetics 123:281-292