CC-3794 sac1::ARG7 nit1 mt+ [ars5-4]
$30.00
From John Davies, Iowa State University, August 2000
Phenotype: altered sulfur metabolism
Derived from transformation of CC-425 with plasmid pJD76, subsequently crossed with CC-2677 (nit1-305).
This strain does not express arylsulfatase activity when grown under sulfur-deficient conditions. As a group, the sac1 mutants fail to accumulate arylsulfatase or the proteins of the high-affinity sulfate transport system when transferred to sulfur-deficient medium. They are also unable to decrease photosynthesis under these conditions, with the result that they die when cultured in the light under sulfur-limiting conditions.
Davies JP, Yildiz F, Grossman AR (1994) Mutants of Chlamydomonas with Aberrant Responses to Sulfur Deprivation. Plant Cell 6:53-63
Davies JP, Yildiz FH, Grossman A (1996) Sac1, a putative regulator that is critical for survival of Chlamydomonas reinhardtii during sulfur deprivation. EMBO J 15:2150-2159
Moseley JL, Gonzalez-Ballester D, Pootakham W, Bailey S, Grossman AR (2009) Genetic interactions between regulators of Chlamydomonas phosphorus and sulfur deprivation responses. Genetics 181:889-905