* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-3861 fla15 mt-

Name: CC-3861 fla15 mt-
SKU: CC-3861
Availability: InStock

$30.00

From Gianni Piperno, Mount Sinai School of Medicine, June 2001

Phenotype: impaired motility

The fla15 mutant was isolated by Piperno in the 137c background, after mutagenesis with nitrosoguanidine. It shows temperature-conditional flagellar assembly, and is defective in retrograde intraflagellar transport. Iomini et al. found that the mutation is a missense mutation in a conserved cysteine in the IFT144 gene.

Piperno G, Siuda E, Henderson S, Segil M, Vaananen H, Sassaroli M (1998) Distinct mutants of retrograde intraflagellar transport (IFT) share similar morphological and molecular defects. J Cell Biol 143:1591-1601

Iomini C, Li L, Esparza JM, Dutcher SK (2009) Retrograde intraflagellar transport mutants identify complex A proteins with multiple genetic interactions in Chlamydomonas reinhardtii. Genetics 183:885-896

Locus:
FLA15 [IFT144]
Chromosome:
13

CC-3861 fla15 mt-

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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