CC-3862 fla17-3 (fla16-1) mt+
$30.00
From Gianni Piperno, Mount Sinai School of Medicine, June 2001
Phenotype: impaired motility
The fla16 mutant was isolated by Piperno in the 137c background, after mutagenesis with nitrosoguanidine. It showed temperature-conditional flagellar assembly. The original fla16 appeared to be unlinked to fla17 (Iomini et al., 2001) but in a later study (2009) Iomini et al. found that the mutant deposited in the stock collection did not recombine with fla17-1. They therefore renamed this strain fla17-3.
Piperno G, Siuda E, Henderson S, Segil M, Vaananen H, Sassaroli M (1998) Distinct mutants of retrograde intraflagellar transport (IFT) share similar morphological and molecular defects. J Cell Biol 143:1591-1601
Iomini C, Babaev-Khaimov V, Sassaroli M, Piperno G (2001) Protein particles in Chlamydomonas flagella undergo a transport cycle consisting of four phases. J Cell Biol 153:13-24
Iomini C, Li L, Esparza JM, Dutcher SK (2009) Retrograde intraflagellar transport mutants identify complex A proteins with multiple genetic interactions in Chlamydomonas reinhardtii. Genetics 183:885-896