CC-3872 pg-113 arg2 mt+
$30.00
From Arminio Boschetti, University of Bern, August 2001
Phenotype: chlorophyll and carotenoid deficient; requires arginine
From Boschetti’s notes on this strain: The pg-113 mutant was isolated by M. Tellenbach in Boschetti’s laboratory, by selecting cells with altered color after UV mutagenesis of an arg2 strain (CCAP 11/32f, Levine arg2 mt+) and subsequently determined in collaboration with Alexander Chunayev to be an allele at the CBN1 locus although its phenotype differs somewhat from the cbn1-48 mutant that defines that locus. It lacks a stable chlorophyll complex and chlorophyll b, but has normal chlorophyll a., and the LHCII proteins are normal. The amount of neoxanthin is reduced. This strain grows phototrophically but should be kept at low to medium light intensity.
Michel H, Tellenbach M, Boschetti A (1983) A chlorophyll b-less mutant of Chlamydomonas reinhardii lacking in the light-harvesting chlorophyll complex but not in its apoproteins. Biochim Biophys Acta 725:417-424
Hoober JK, Maloney MA, Asbury LR, Marks DB (1990) Accumulation of Chlorophyll a/b-Binding Polypeptides in Chlamydomonas reinhardtii y-1 in the Light or Dark at 38 degrees C : Evidence for Proteolytic Control. Plant Physiol 92:419-426
Hoober JK, Hughes MJ (1992) Purification and Characterization of a Membrane-Bound Protease from Chlamydomonas reinhardtii. Plant Physiol 99:932-937