* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-3883 RbcS2 2-1aa III
$30.00
From Arminio Boschetti, University of Bern, August 2001
CC-3875 through CC-3893 are a collection of transformants of the T60-3 Rubisco knockout mutant created by Khrebtukova and Spreitzer (CC-4415).
For CC-3883, the plasmid used for the transformation leading to CC-3875 was modified by mutating processing site 2 in the transit peptide of the precursor of the Rubisco small subunit such that Met(46) is exchanged by His(46) and a NdeI restriction site is introduced into the DNA. Transformation resulted in complementation of rbcS-T60-3 to normal photoautotrophy. Synthesis of intermediate sized small subunit is enhanced, but mature small subunit is also formed.