* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-3890 RbcS2 2-2aa VII

Name: CC-3890 RbcS2 2-2aa VII
SKU: CC-3890
Availability: InStock

$30.00

From Arminio Boschetti, University of Bern, August 2001

CC-3875 through CC-3893 are a collection of transformants of the T60-3 Rubisco knockout mutant created by Khrebtukova and Spreitzer (CC-4415).

For CC-3890, the plasmid used for the transformation leading to CC-3875 was modified by mutating processing site 2 in the transit peptide of the precursor of the Rubisco small subunit such that Gln(45)/Met(46) are exchanged by Ile(45)/His(46) and a NdeI restriction site is introduced into the DNA. Transformation resulted in complementation of rbcS-T60-3 to photoautotrophy, but strains grow more slowly. Formation of intermediate sized small subunit is very much enhanced. No mature small subunit was detected by Western blotting, but some immuno cross-reacting proteins of slightly higher molecular masses were detected.

Locus:
RBCS2
Chromosome:

CC-3890 RbcS2 2-2aa VII

Products

Support

Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

Cart