* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-3947 nic7 thi10 MID mt(+)-
$30.00
From Patrick Ferris, Washington University, April 2002, his strain K33
Phenotype: requires nicotinamide and thiamine
An imp1 arg2 strain (CC-1865) was transformed with pArg7.8 and a 3.5 kb ApaI fragment containing the MID gene. One of these transformants (B28) was crossed to CC-85 (nic7 mt+) to create a nic7 mt+ strain with a MID transgene. This strain was then crossed to CC-123 (thi10 mt+ ) to create the strains called K33 and L21 (see CC-3948). K33 retains the MID transgene, and although genetically plus, it mates as minus.