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CC-4010 osm9 mt+

Name: CC-4010 osm9 mt+
SKU: CC-4010
Availability: InStock

$30.00

From Peter Luykx, University of Miami, January 2004, his PL52

Phenotype: deficient in osmoregulation

The osm9 mutant was obtained in 1995 in the laboratory of Greg Pazour, by insertional mutagenesis of a nit1-305 strain originally derived from CC-2453, using a NIT1-containing plasmid pGP505, but the NIT phenotype does not cosegregate with the osm phenotype. Please see CC-4064 for another osm9 isolate that apparently lacks the NIT1 insertion. CC-4010 was obtained by backcrossing the original mutant three times to wild type CC-124 or CC-125 and re-isolating. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

Luykx P, Hoppenrath M, Robinson DG (1997) Osmoregulatory mutants that affect the function of the contractile vacuole in Chlamydomonas reinhardtii. Protoplasma 200:99-111

CC-4010 osm9 mt+

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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