CC-4039 CR39 (amt4) mt+
$30.00
From William Inwood and Sydney Kustu, UC Berkeley, January 2005
Phenotype: inhibitor resistant (methylammonium)
This is a spontaneous methylammonium-resistant mutant isolated in the 4A+ background (CC-4051). The CR39 mutant carries a 300-bp deletion of portions of exon 5 and intron 5 of the AMT4 gene. Assuming normal splicing of the remaining transcript, this lesion leads to translation of a truncated protein of 385 correct amino acids (terminating within predicted transmembrane segment 10) followed by nine incorrect amino acids from intron 5 sequence. The lesion creates an Amt4 null phenotype.
Kim KS, Feild E, King N, Yaoi T, Kustu S, Inwood W (2005) Spontaneous mutations in the ammonium transport gene AMT4 of Chlamydomonas reinhardtii. Genetics 170:631-644