CC-4040 CR40 (amt4) mt+
$30.00
From William Inwood and Sydney Kustu, UC Berkeley, January 2005
Phenotype: inhibitor resistant (methylammonium)
This is a spontaneous methylammonium-resistant mutant isolated in the 4A+ background (CC-4051). The CR40 mutant carries a large insert near the end of exon 1 of the AMT4 gene, which was identified by Kim et al. as a TOC1 transposon. Low residual transcript levels for cells grown on arginine (3′ end of AMT4) were not elevated above those for cells grown on ammonium, and Kim et al. concluded that transcription of the 3′ end of AMT4 is probably being driven from within TOC1. The lesion in CR40 is an amt4 null allele.
Kim KS, Feild E, King N, Yaoi T, Kustu S, Inwood W (2005) Spontaneous mutations in the ammonium transport gene AMT4 of Chlamydomonas reinhardtii. Genetics 170:631-644