CC-41 cr1 mt+
$30.00
Boynton-Gillham laboratory, Duke University
Phenotype: cold sensitive on minimal medium
Product 5.6.3 from a cross of wild type to the original ac20 cr1 double mutant (CC-154 mt-, a strain now discontinued.)
This strain was used by Boynton et al. (1972) in their study of mutants deficient in chloroplast ribosomes, and defines the cr1 mutation in terms of chloroplast ribosome content (accumulation of 54S subunits). This strain now shows a ribosome profile on sucrose gradients more like that of the ac20 cr1 double mutant. It grows slightly on minimal medium. Scoring is easier at cool temperatures, e.g. 15 degrees.
Boynton JE, Gillham NW, Chabot JF (1972) Chloroplast ribosome deficient mutants in the green alga Chlamydomonas reinhardi and the question of chloroplast ribosome function. J Cell Sci 10:267-305
Harris EH, Boynton JE, Gillham NW (1974) Chloroplast ribosome biogenesis in Chlamydomonas. Selection and characterization of mutants blocked in ribosome formation. J Cell Biol 63:160-179