* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-4133 VFL1-2-R2
$30.00
From Carolyn Silflow, University of Minnesota, January 2007
VFL1-2-R2 is a rescued strain with wild-type motility obtained by cotransformation of vfl1-2, arg7 with pARG7.8 and an epitope-tagged construct of the VFL1 gene, pW6-10.0-3HA. The VFL1 gene product can be detected with antibodies against the HA epitope tag, as described by Silflow et al. 2001.
For more information on the VFL1 locus, please see CC-1388.
Silflow CD, LaVoie M, Tam LW, Tousey S, Sanders M, Wu W, Borodovsky M, Lefebvre PA (2001) The Vfl1 Protein in Chlamydomonas localizes in a rotationally asymmetric pattern at the distal ends of the basal bodies. J Cell Biol 153:63-74