CC-4147 FUD7 (psbA deletion) mt+
$30.00
From Jacqueline Girard-Bascou, Institut de Biologie Physico-Chimique, January 2007
Phenotype: requires acetate
This strain replaces CC-741 FUD7 mt+, which has been widely used as a recipient for chloroplast transformation. This is a deletion mutation spanning the psbA gene, and it can be rescued by chloroplast DNA clones containing the wild type gene.
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Wollman FA, Olive J, Bennoun P, Recouvreur M (1980) Organization of the photosystem II centers and their associated antennae in the thylakoid membranes: a comparative ultrastructural, biochemical, and biophysical study of Chlamydomonas wild type and mutants lacking in photosystem II reaction centers. J Cell Biol 87:728-735
Bennoun P, Spierer-Herz M, Erickson J, Girard-Bascou J, Pierre Y, Delosme M, Rochaix JD (1986) Characterization of photosystem II mutants of Chlamydomonas reinhardii lacking the psbA gene. Plant Mol Biol 6:151-160
Minagawa J, Crofts AR (1994) A robust protocol for site-directed mutagenesis of the D1 protein inChlamydomonas reinhardtii: A PCR-splicedpsbA gene in a plasmid conferring spectinomycin resistance was introduced into apsbA deletion strain. Photosynth Res 42:121-131