CC-4148 FUD16 (atpA) mt+
$30.00
From Jacqueline Girard-Bascou, Institut de Biologie Physico-Chimique, January 2007
Phenotype: requires acetate
This mutant was isolated by Bennoun after treatment with 5-fluorodeoxyuridine. Ketchner et al. showed that FUD16 contains two missense mutations in the atpA gene, changing Ile at 184 to Asn and Asn at 186 to Tyr in a strictly conserved region downstream of the P-loop. The mutant is acetate-requiring and deficient in ATP synthase activity, but over-produces the alpha and beta subunits of the chloroplast CF1 ATP synthase complex.
Delepelaire P, Bennoun P (1978) Energy transfer and site of energy trapping in photosystem I. Biochim Biophys Acta 502:183-187
Woessner JP, Masson A, Harris EH, Bennoun P, Gillham NW, Boynton JE (1984) Molecular and genetic analysis of the chloroplast ATPase of chlamydomonas. Plant Mol Biol 3:177-190
Lemaire C, Wollman FA (1989) The chloroplast ATP synthase in Chlamydomonas reinhardtii. II. Biochemical studies on its biogenesis using mutants defective in photophosphorylation. J Biol Chem 264:10235-10242
Ketchner SL, Drapier D, Olive J, Gaudriault S, Girard-Bascou J, Wollman FA (1995) Chloroplasts can accommodate inclusion bodies. Evidence from a mutant of Chlamydomonas reinhardtii defective in the assembly of the chloroplast ATP synthase. J Biol Chem 270:15299-15306